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Abstract9 D6 C+ G/ ?. z6 Y( ?% C( {
Aligned microcontact printing for patterning the sample in areas of homogeneous RF-field on the highly sensitive surface of planar
) ]: ]1 @4 f& y( g( `7 C. H* b6 qNMR microprobes is presented. We experimentally demonstrate that sample patterning allows drastic improvement of the spin excitation
) M* b; {- V8 ?5 Nuniformity. The NMR microprobes are designed for cell analysis and characterized using lipid vesicles as cell substitutes. Lipid vesicles, ^9 a( l* u" R
are advantageous as composition and concentration of the confined solution are precisely controlled and because of their similarity
: {3 Z) f5 o7 s- Nto living cells. Using aligned microcontact printing, a monolayer of lipid vesicles is immobilized on the surface of the planar NMR microprobe& {3 l+ c6 U$ i
in a patterned way. 1H NMR spectra and CPMG spin echoes of sucrose solution confined within the lipid vesicles are successfully; J- x8 s- u) \
recorded. Nutation curves of the sample structured in different patterns demonstrate the impact of patterning on the spin excitation uniformity.5 [2 q! y2 z( d$ a8 K
The total detection volumes are between 1 and 2 nL and derived with help of a theoretic model based on 3D finite element simulation.1 o5 n% x O& I# P5 g2 Y
This model predicts the signal-to-noise ratio and the progression of the nutation curves.9 p' s9 v- P3 M! ]4 n, P2 Z& L3 _
2005 Elsevier Inc. All rights reserved.
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[ 本帖最後由 mt7344 於 2007-6-7 10:38 PM 編輯 ] |
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