|
Abstract9 m) ?4 D0 ]* N
Aligned microcontact printing for patterning the sample in areas of homogeneous RF-field on the highly sensitive surface of planar
, _ e Z$ ^0 s% k( XNMR microprobes is presented. We experimentally demonstrate that sample patterning allows drastic improvement of the spin excitation6 h& |. b$ G, s/ M: w$ G$ v
uniformity. The NMR microprobes are designed for cell analysis and characterized using lipid vesicles as cell substitutes. Lipid vesicles; v- \& B2 K8 |) Z
are advantageous as composition and concentration of the confined solution are precisely controlled and because of their similarity
$ M ]9 W( t7 I" o/ rto living cells. Using aligned microcontact printing, a monolayer of lipid vesicles is immobilized on the surface of the planar NMR microprobe' K( B* }: z& B h0 i
in a patterned way. 1H NMR spectra and CPMG spin echoes of sucrose solution confined within the lipid vesicles are successfully5 t# ?# R" V! q( } l1 h
recorded. Nutation curves of the sample structured in different patterns demonstrate the impact of patterning on the spin excitation uniformity.; d+ ~- i, k0 C
The total detection volumes are between 1 and 2 nL and derived with help of a theoretic model based on 3D finite element simulation.
* l+ G7 {9 }2 `4 N1 ~This model predicts the signal-to-noise ratio and the progression of the nutation curves.
, U( ~. L, m' _& ] 2005 Elsevier Inc. All rights reserved.
1 ^; t. r B, e' N% \* }; M" K" W. R; t4 K8 N
8 H" i( e& W9 R# O
網路上抓的 paper, 希望對大家有幫助!!
& e- {* R: k9 o3 R/ }2 Z; x+ V權限10 & RDB=38 h2 O5 O0 T, k7 s
3 l! _' Z" B8 w
- H# F% Q# R+ I5 \6 l6 h' G' b$ O ]9 a$ s
8 R O2 @. [- T0 b6 B" _2 d5 `, J[ 本帖最後由 mt7344 於 2007-6-7 10:38 PM 編輯 ] |
本帖子中包含更多資源
您需要 登錄 才可以下載或查看,沒有帳號?申請會員
x
|