Sample patterning on NMR surface microcoils[EPFL]

mt7344

Abstract
Aligned microcontact printing for patterning the sample in areas of homogeneous RF-field on the highly sensitive surface of planar
NMR microprobes is presented. We experimentally demonstrate that sample patterning allows drastic improvement of the spin excitation
3 ?% w# {* Z" p* Buniformity. The NMR microprobes are designed for cell analysis and characterized using lipid vesicles as cell substitutes. Lipid vesicles
( ~& s' p/ _/ j4 Yare advantageous as composition and concentration of the confined solution are precisely controlled and because of their similarity
3 i( D: ?* R8 V/ H( O& uto living cells. Using aligned microcontact printing, a monolayer of lipid vesicles is immobilized on the surface of the planar NMR microprobe
in a patterned way. 1H NMR spectra and CPMG spin echoes of sucrose solution confined within the lipid vesicles are successfully
recorded. Nutation curves of the sample structured in different patterns demonstrate the impact of patterning on the spin excitation uniformity.
The total detection volumes are between 1 and 2 nL and derived with help of a theoretic model based on 3D finite element simulation.
This model predicts the signal-to-noise ratio and the progression of the nutation curves.

2005 Elsevier Inc. All rights reserved.

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